Search results for "methods [Thrombolytic Therapy]"

Distribution Amplitudes of Heavy-Light Mesons

2019

A symmetry-preserving approach to the continuum bound-state problem in quantum field theory is used to calculate the masses, leptonic decay constants and light-front distribution amplitudes of empirically accessible heavy-light mesons. The inverse moment of the $B$-meson distribution is particularly important in treatments of exclusive $B$-decays using effective field theory and the factorisation formalism; and its value is therefore computed: $\lambda_B(\zeta = 2\,{\rm GeV}) = 0.54(3)\,$GeV. As an example and in anticipation of precision measurements at new-generation $B$-factories, the branching fraction for the rare $B\to \gamma(E_\gamma) \ell \nu_\ell$ radiative decay is also calculated…

Illumina-based RiboMethSeq approach for mapping of 2'-O-Me residues in RNA

2016

International audience; RNA 2'-O-methylation is one of the ubiquitous nucleotide modifications found in many RNA types from Bacteria, Archaea and Eukarya. RNAs bearing 2'-O-methylations show increased resistance to degradation and enhanced stability in helices. While the exact role of each 2'-O-Me residue remained elusive, the catalytic protein Fibrillarin (Nop1 in yeast) responsible for 2'-O-methylation in eukaryotes, is associated with human pathologies. Therefore, there is an urgent need to precisely map and quantify hundreds of 2'-O-Me residues in RNA using high-throughput technologies. Here, we develop a reliable protocol using alkaline fragmentation of total RNA coupled to a commonly …

SM identification of approximating models forH∞ robust control

1999

Set Membership (SM) W, identification of mixed parametric and nonparametric models is investigated, aimed to estimate a low order approximating model and an identification error, giving a measure of the unmodeled dynamics in a form well suited for H, control methodologies. In particular, the problem of estimating the parameters of the parametric part and the H, bound on the modeling error is solved using frequency domain data, supposing lbo bounded measurement errors and exponentially stable unmodeled dynamics. The effectiveness of the proposed procedure is tested on some numerical examples, showing the advantages of the proposed methods over the existing nonparametric H, identification app…

Biopsy vs. brushing: comparison of two sampling methods for the detection of HPV-DNA in squamous cell carcinoma of the oral cavity

2012

Background: HR HPV infection was proposed as aetiological factor of oral squamous cell carcinomas (OSCC). HPV frequency in OSCC is highly variable, due to the discrepancy in oral sampling procedures, HPV testing methods and inclusion criteria regarding tumour site (strictly oral cavity vs. nearby structures). Our aim was to compare HPV DNA frequency and type-specific distribution in paired cytological and histological samples of SCC strictly located in oral cavity. The correlation between HPV detection rate by each method of sampling and demographical, behavioural and clinical-pathological variables was also examined. Patients and methods: HPV DNA was detected in brushed cells and formalin-…

The contribution of colour and spatial frequency cues to contour integration

2011

Beobachtungen nach einer neuen optischen Methode: ein Beitrag zur Experimental-Physik

1864

Inhalt: Princip der Beobachtungs-Methode... 2 Specielle Beschreibung des Apparates... 8 Bemerkungen über den Gebrauch und die Adjustirung... 13 Schlieren in Gläsern ... 16 Beobachtungen mittels des Schlieren-Apparates... 18 Veränderung durchsichtiger Körper durch Temperatur und Druck... 19 Diffusionsbewegungen... 21 Untersuchung der Flammen... 23 Beugungsphänomene... 26 Sichtbarmachung der Schallwellen... 27 Beobachtungen über den electrischen Funken... 29 Der Inductionsfunken... 35 Der Flaschenfunken... 42 Schlussbemerkungen... 49

Direct measurement of the melting temperature of supported DNA by electrochemical method

2003

The development of biosensors based on DNA hybridization requires a more precise knowledge of the thermodynamics of the hybridization at a solid interface. In particular, the selectivity of hybridization can be affected by a lot of parameters such as the single-strand (ss)DNA density, the pH, the ionic strength or the temperature. The melting temperature, T(m), is in part a function of the ionic strength and of the temperature and therefore provides a useful variable in the control of the selectivity and sensitivity of a DNA chip. The electrochemical technique has been used to determine the T(m) values when the probe is tethered by a DNA self-assembled monolayer (SAM). We have built a speci…

La distribuzione di Maxwell-Boltzmann: i risultati di una sperimentazione svolta nell'ambito dei corsi di laurea in ingegneria

2011

This paper describes the outcomes of an teaching experiment conducted in the seminar held at the Faculty Engineering of Palermo University. The theme of the seminar focused on undergraduate experiment that yields the velocity distribution of thermionic electrons by analyzing the I-V characteristics of diodes and triodes. By using a simple model, the velocity distribution of thermionic electrons emitted by the vacuum tube cathode can be described by Maxwell's distribution. The experiment allows students to focus on the distribution function more than on difficulties arising from the complexity of thermionic emission. Questo articolo descrive i risultati di una sperimentazione didattica condo…

A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization

2009

We describe a quantitative method for detecting RNA alternative splicing variants that combines in situ hybridization of fluorescently labeled peptide nucleic acid (PNA) probes with confocal microscopy Förster resonance energy transfer (FRET). The use of PNA probes complementary to sequences flanking a given splice junction allows to specifically quantify, within the cell, the RNA isoform generating such splice junction by FRET measure. As a proof of concept we analyzed two alternative splicing events originating from lymphocyte antigen 6 (LY6) complex, locus G5B (LY6G5B) pre-mRNA. These are characterized by the removal of the first intron (Fully Spliced Isoform, FSI) or by retention of suc…

'School adopts an experiment': the magnetic levitation of superconductors

2010

The event 'School adopts an experiment' is an event targeted at high schools and secondary schools. It is based on a tight and direct collaboration between researchers and school students and teachers. Several schools were involved in the event by 'adopting' an experiment in physics research laboratories. Groups of selected students were first trained by university researchers, then they demonstrated the experiments to other students and teachers during the event. Students who adopted an experiment acquired knowledge of the subjects connected with the experiment in a most impressive way compared with the usual didactics at school. Further positive points were: (i) to establish a close and d…